Accessing genes in the tertiary gene pool of rice by direct introduction of total DNA from Zizania palustris (Wild Rice)
Abedinia, M, Henry, RJ, Blakeney, AB & Lewin, LG 2000, 'Accessing genes in the tertiary gene pool of rice by direct introduction of total DNA from Zizania palustris (Wild Rice)', Plant Molecular Biology Reporter, vol. 18, no. 2, pp. 133-138.
The original publication is available at www.springerlink.com at http://dx.doi.org/10.1007/BF02824021
Transfer of useful genes from wild relatives of crop plants has relied upon successful conventional crossing or the availability of the cloned gene. Co-bombardment of rice callus with total genomic DNA from wild rice (Zizania palustris) and a plasmid containing a gene confirming hygromycin resistance allowed recovery under selection of transgenic plants with grain characteristics from wild rice. Amplified Fragment Length Polymorphism (AFLP) analysis suggested that a significant amount of DNA from Zizania was introduced by this procedure. One plant had 16 of a possible 122 Zizania specific AFLP markers detected with the primers used. This approach may have potential for introgression of genes from wild relatives in other cases where highly efficient transformation methods are available.