PCR based molecular marker for the fragrance gene in rice (Oryza sativa. L)
Garland, SH, Lewin, LG, Blakeney, AB, Reinke, RF & Henry, RJ 2000, 'PCR based molecular marker for the fragrance gene in rice (Oryza sativa. L)', Theoretical & Applied Genetics, vol. 101, no. 3, pp. 364-371.
Published version available from: http://dx.doi.org/10.1007/s001220051492
The genomic DNA clone RG28, linked to the major fragrance gene of rice (fgr), was assessed for polymorphism in order to produce a PCR-based marker for fragrance. A small mono-nucleotide repeat, that was polymorphic between a pair of fragrant and non-fragrant cultivars, was identified and developed into a co-dominant PCR-based marker. The polymorphism-information-content determinations for three microsatellite markers, that have been genetically mapped near RG28, are also presented. These PCR-based markers will be highly useful in distinguishing fragrance-producing alleles from non-fragrance-producing alleles at the fgr locus.