Fast resolution of identification problems in seed production and plant breeding using molecular markers
Poulsen, DME, Ko, HL, van der Meer, JG, van der Putte, PM & Henry, RJ 1996, 'Fast resolution of identification problems in seed production and plant breeding using molecular markers', Australian Journal of Experimental Agriculture, vol. 36, no. 5, pp. 571-576.
The original publication is available at www.publish.csiro.au at http://dx.doi.org/10.1071/EA9960571
Polymerase chain reaction (PCR) analysis with specific and arbitrary primers was used to identify germplasm from a barley breeding program. Through practical application of the technology, an F1 plant's identity was confirmed, the correct pedigrees 'of 2 incorrectly labelled F2 populations were determined, confusion in the identity of seed lots of an advanced breeding line was resolved and off-type barley plants were identified in a seed increase block. This illustrated the value of PCR-based genotype analysis in plant breeding programs.