Title

Optimisation of a microsatellite enrichment technique in saccharum spp.

Document Type

Article

Publication details

Cordeiro, GM, Maguire, TL, Edwards KJ & Henry, RJ 1999, 'Optimisation of a microsatellite enrichment technique in saccharum spp.', Plant Molecular Biology Reporter, vol. 17, no. 3, pp. 225-229.

The original publication is available at www.springerlink.com, http://dx.doi.org/10.1023/A:1007640231900

Peer Reviewed

Peer-Reviewed

Abstract

The creation of enriched microsatellite libraries can ensure an abundant supply of microsatellite sequences at a considerably reduced cost. We report here the modification to an existing enrichment protocol and the optimisation of this technique for use on specific plant species, in this case, sugarcane. Using this optimised protocol, we achieved a 40% increase in the level of enrichment.