Title

The use of microsatellite polymorphisms for the identification of Australian breeding lines of rice (Oryza sativa L.)

Document Type

Article

Publication details

Garland, SH, Lewin LG, Abedinia, M, Henry, RJ & Blakeney, A 1999, 'The use of microsatellite polymorphisms for the identification of Australian breeding lines of rice (Oryza sativa L.)', Euphytica, vol. 108, no. 1, pp. 53-63.

The original publication is available at www.springerlink.com, http://dx.doi.org/10.1023/A:1003688612179

Peer Reviewed

Peer-Reviewed

Abstract

Ten microsatellite loci were analysed for 43 cultivars or breeding lines of rice. Polymorphism-Information-Content values ranged from 0.62 to 0.92. The microsatellite markers were found to be useful for cultivar identification and assessment of genetic relationships. Most of the cultivars could be uniquely identified by at least one microsatellite marker. Genetic heterogeneity was detected within rice samples by amplification of microsatellites from DNA extracted from multiple individual plants and also from bulked DNA preparations.