Title

Phthalhydrazide chemiluminescence method for determination of hydroxyl radical production: modifications and adaptations for use in natural systems

Document Type

Article

Publication details

Miller, CJ, Rose, AL & Waite, TD 2011, 'Phthalhydrazide chemiluminescence method for determination of hydroxyl radical production: modifications and adaptations for use in natural systems', Analytical Chemistry, vol. 83, no. 1, pp. 261-268.

Publisher version available from: http://dx.doi.org/10.1021/ac1022748

Peer Reviewed

Peer-Reviewed

Abstract

The hydroxyl radical is formed through a variety of processes pertinent to natural and anthropogenic systems. Here we report development of a simple and sensitive trap-and-trigger chemiluminescence method based upon the hydroxylation of phthalhydrazide to 5-hydroxy-2,3-dihydro-1,4-phthalazinedione, which emits chemiluminescence when oxidized under alkaline conditions. Cu(III) is employed as an oxidant and is shown to be relatively insensitive to a range of interferences likely to be encountered. The method has been standardized by use of