Title

Comparison of two methods for the isolation of phytolith occluded carbon from plant material

Document Type

Article

Publication details

Parr, JF & Sullivan, LA 2014 , 'Comparison of two methods for the isolation of phytolith occluded carbon from plant material, Plant and Soil, vol. 374, no. 1-2, pp. 45-53.

Published version available from:

http://doi.org/10.1007/s11104-013-1847-1

Peer Reviewed

Peer-Reviewed

Abstract

Background and aims

Phytolith occluded carbon (PhytOC) is of interest for isotope studies, dating of sediments and the capture and storage of carbon. Many methodologies have been used for the isolation of phytoliths from plant material; however, there are wide disparities in the PhytOC contents when determined by different methodologies. In this study we examine the utility of the two main methods used for quantifying PhytOC.

Methods

These methods are: (1) a microwave digestion followed by a Walkley-Black digestion, and (2) H2SO4/H2O2.

Results

Method (1) produced PhytOC values over 50 times higher than those acquired by method (2). SEM examination indicated that the differences were likely due to shattering of the phytoliths by method (2) allowing consumption by the acid and peroxide of PhytOC .

Conclusion

These results indicate that for the samples analysed here: 1] the modified microwave method allowed the total PhytOC to be measured, 2] the H2SO4/H2O2 method allowed the PhytOC within the tightly packed silica matrix to be measured, and 3] the PhytOC retained within the phytolith cavities could possibly be calculated by subtracting 2] from 1]. For the samples analysed here most of the PhytOC resided in the phytolith cavities.