Development of a method for the separation of corrinoids in ovine tissues by HPLC
Kelly, RJ, Gruner, TM & Sykes, AR 2005, 'Development of a method for the separation of corrinoids in ovine tissues by HPLC', Biomedical Chromatography, vol. 19, no. 4, pp. 329-333.
The publisher's version of this article is available at http://dx.doi.org/10.1002/bmc.444
A method has been developed using a combination of high-performance liquid chromatography (HPLC) and a radioisotope dilution assay (RIDA) to routinely estimate the distribution of corrinoids (the cobalamins hydroxocobalamin, methylcobalamin and 5-deoxyadenosylcobalamin, and cobalamin analogues) in liver, plasma, milk, intestinal fluid and faeces. Corrinoids were extracted with a sodium acetate buffer, separated by HPLC and quantified by RIDA. Recoveries of corrinoids were 29% for hydroxocobalamin, 50% for 5-deoxyadenosylcobalamin and 64% for methylcobalamin. The method allows the routine analysis of many samples and maintains good standards of precision.