Title

Sesquiterpene lactone containing Mexican Indian medicinal plants and pure sesquiterpene lactones as potent inhibitors of transcription factor NF-κB

Document Type

Article

Publication details

Bork, PM, Schmitz, ML, Kuhnt, M, Escher, C & Heinrich, M 1997, 'Sesquiterpene lactone containing Mexican Indian medicinal plants and pure sesquiterpene lactones as potent inhibitors of transcription factor NF-κB', FEBS Letters, vol. 402, no. 1, pp. 85-90.

The publisher's version of this article available at http://dx.doi.org/10.1016/S0014-5793(96)01502-5

Peer Reviewed

Peer-Reviewed

Abstract

The potential inhibitory effect of 54 Mexican Indian medicinal plants on the activation of transcription factor NF-κB was studied. Band-shift experiments identified the ethanolic leaf extracts of Artemisia ludoviciana ssp. mexicana, Calea zacatechichi, and Polymnia maculata (all rich in sesquiterpene lactones) as inhibitors of NF-κB down to a concentration of 25 μg/ml. The sesquiterpene lactones isohelenin and parthenolide prevented NF-κB activation completely as low as 5 μM. Treatment of HeLa cells with leaf extract of A. ludoviciana ssp. mexicana, isohelenin and parthenolide prevented the induction of transcription on the IL-6 promoter. These experiments identify the eudesmanolide and germacranolide type of sesquiterpene lactones as potent non-antioxidant inhibitors of NF-κB. All plants active in the NF-κB assay also showed a delay in the onset of capillary reactions of the allantois membrane in a physiological model for anti-inflammatory activity - the HET-CAM assay.