Title

Stability, structure and complexity of yeast chromosome-III

Document Type

Article

Publication details

King, GJ 1993, 'Stability, structure and complexity of yeast chromosome-III', Nucleic Acids Research, vol. 21, no. 18, pp. 4239-4245.

Published version available from:

http://dx.doi.org/10.1093/nar/21.18.4239

Peer Reviewed

Peer-Reviewed

Abstract

The complete sequence of yeast chromosome III provides a model for studies relating DNA sequence and structure at different levels of organisation in eukaryotic chromosomes. DNA helical stability, intrinsic curvature and sequence complexity have been calculated for the complete chromosome. These features are compartmentalised at different levels of organisation. Compartmentallsatlon of thermal stability Is observed from the level delineating coding/noncoding sequences, to higher levels of organisation which correspond to regions varying In G + C content. The three-dimensional path reveals a symmetrical structure for the chromosome, with a densely packed central region and more diffuse and linear subtelomeric regions. This interspersion of regions of high and low curvature Is reflected at lower levels of organisation. Complexity of n-tuplets (n = 1 to 6) also reveals compartmentallsation of the chromosome at different levels of organisation, in many cases corresponding to the structural features. DNA stability, conformation and complexity delineate telomeres, centromere, autonomous replication sequences (ARS), transposition hotspots, recombination hotspots and the mating-type loci.