Title

Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers

Authors

C Maliepaard, DLO-Centre for Plant Breeding and Reproduction Research, The Netherlands
F H. Alston, Horticulture Research International, UK
G van Arkel, DLO-Centre for Plant Breeding and Reproduction Research, The Netherlands
L M. Brown, Horticulture Research International, UK
E Chevreau, Centre de Recherches dÕAngers, France
F Dunemann, Institute for Ornamental Plant Breeding, Germany
K M. Evans, Horticulture Research International, UK
S Gardiner, Horticulture and Food Research Institute of New Zealand, Palmerston North
P Guilford, Horticulture and Food Research Institute of New Zealand, Auckland
A W. van Heusden, DLO-Centre for Plant Breeding and Reproduction Research, The Netherlands
J Janse, DLO-Centre for Plant Breeding and Reproduction Research, The Netherlands
F Laurens, Centre de Recherches dÕAngers, France
J R. Lynn, Horticulture Research International, UK
A G. Manganaris, NAGREF, Greece
A PM den Nijs, DLO-Centre for Plant Breeding and Reproduction Research, The Netherlands
N Periam, Horticulture Research International, UK
E Rikkerink, Horticulture and Food Research Institute of New Zealand, Auckland
P Roche, Horticulture Research International, UK
C Ryder, Horticulture Research International, UK
S Sansavini, University of Bologna, Italy
H Schmidt, Institute for Ornamental Plant Breeding, Germany
S Tartarini, University of Bologna, Italy
J J. Verhaegh, DLO-Centre for Plant Breeding and Reproduction Research, The Netherlands
M Vrielink-van Ginkel, DLO-Centre for Plant Breeding and Reproduction Research, The Netherlands
Graham J. King, Southern Cross UniversityFollow

Document Type

Article

Publication details

Maliepaard, C, Alston, FH, van Arkel, G, Brown, LM, Chevreau, E, Dunemann, F, Evans, KM, Gardiner, S, Guilford, P, van Heusden, AW, Janse, J, Laurens, F, Lynn, JR, Manganaris, AG, den Nijs, APM, Periam, N, Rikkerink, E, Roche, P, Ryder, C, Sansavini, S, Schmidt, H, Tartarini, S, Verhaegh, JJ, Vrielink-van Ginkel, M & King, GJ 1998, 'Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers', Theoretical and Applied Genetics, vol. 97, no. 1-2, pp. 60-73.

Published version available from:

http://dx.doi.org/10.1007/s001220050867

Peer Reviewed

Peer-Reviewed

Abstract

Linkage maps for the apple cultivars ‘Prima’ and ‘Fiesta’ were constructed using RFLP, RAPD, isozyme, AFLP, SCAR and microsatellite markers in a ‘Prima’�‘Fiesta’ progeny of 152 individuals. Seventeen linkage groups, putatively corresponding to the seventeen haploid apple chromosomes, were obtained for each parent. These maps were aligned using 67 multi-allelic markers that were heterozygous in both parents. A large number of duplicate RFLP loci was observed and, in several instances, linked RFLP markers in one linkage group showed corresponding linkage in another linkage group. Distorted segregation was observed mainly in two regions of the genome, especially in the male parent alleles. Map positions were provided for resistance genes to scab and rosy leaf curling aphid (Vf and Sd 1, respectively) for the fruit acidity gene Ma and for the self-incompatibility locus S. The high marker density and large number of mapped codominant RFLPs and some microsatellite markers make this map an ideal reference map for use in other progenies also and a valuable tool for the mapping of quantitative trait loci.