Title

Calcium ionophoretic and apoptotic effects of ferutinin in the human Jurkat T-cell line

Document Type

Article

Publication details

Machoa, A, Blanco-Molinaa, M, Spagliardib, P, Appendinob, G, Bremnerc, P, Heinrich, M, Fiebichd, BL & Munoz, E 2004, 'Calcium ionophoretic and apoptotic effects of ferutinin in the human Jurkat T-cell line', Biochemical Pharmacology, vol. 68, no. 5, pp. 875-883.

Publishers version of article available at:

http://dx.doi.org/10.1016/j.bcp.2004.05.016

Peer Reviewed

Peer-Reviewed

Abstract

We have investigated the ionophoretic and apoptotic properties of the daucane sesquiterpene ferutinin and three related compounds, ferutidin, 2-α-hydroxyferutidin and teferin, all isolated from various species of plants from the genus Ferula. Ferutinin induced a biphasic elevation of intracellular Ca2+ in the leukemia T-cell line, Jurkat. First, a rapid calcium peak was observed and inhibited by BAPTA-AM. This initial calcium mobilization was followed by a sustained elevation, mediated by the entry of extracellular calcium through L-type calcium channels and sensitive to inhibition by EGTA. Moreover, ferutinin-induced apoptosis in Jurkat cells, and this event was preceded, in a cyclosporine-A sensitive manner, by a loss of mitochondrial transmembrane potential (ΔΨm) and by an increase in intracellular reactive oxygen species. Ferutinin-induced DNA fragmentation was mediated by a caspase-3-dependent pathway, and was initiated independently of any specific phase of the cell cycle. The evaluation of ferutinin analogs in calcium mobilization and apoptosis assays showed strict structure–activity relationships, with p-hydroxylation of the benzoyl moiety being requested for activity.