SNP genotyping allows an in-depth characterisation of the genome of sugarcane and other complex autopolyploids
Garcia, AAF, Mollinari, M, Marconi, TG, Serang, OR, Silva, RR, Vieira, MLC, Vicentini, R, Costa, EA, Mancini, MC, Garcia, MOS, Pastina, MM, Gazaffi, R, Martins, ERF, Dahmer, N, Sforça, DA, Silva, CBC, Bundock, PC, Henry, RJ, Souza, GM, van Sluys, MA, Landell, MGA, Carneiro, MS, Vincentz, MAG, Pinto, LR, Vencovsky, R & Souza, AP 2013, 'SNP genotyping allows an in-depth characterisation of the genome of sugarcane and other complex autopolyploids', Scientific Reports, vol. 3, art. 3399.
Many plant species of great economic value (e.g., potato, wheat, cotton, and sugarcane) are polyploids. Despite the essential roles of autopolyploid plants in human activities, our genetic understanding of these species is still poor. Recent progress in instrumentation and biochemical manipulation has led to the accumulation of an incredible amount of genomic data. In this study, we demonstrate for the first time a successful genetic analysis in a highly polyploid genome (sugarcane) by the quantitative analysis of single-nucleotide polymorphism (SNP) allelic dosage and the application of a new data analysis framework. This study provides a better understanding of autopolyploid genomic structure and is a sound basis for genetic studies. The proposed methods can be employed to analyse the genome of any autopolyploid and will permit the future development of high-quality genetic maps to assist in the assembly of reference genome sequences for polyploid species.