Title

Genome walking

Document Type

Article

Publication details

Shapter, FM & Waters, DLE 2014, 'Genome walking', Methods in Molecular Biology, vol. 1099, pp. 133-146.

Published version available from:

http://dx.doi.org/10.1007/978-1-62703-715-0_12

Abstract

Genome walking is a method for determining the DNA sequence of unknown genomic regions flanking a region of known DNA sequence. The Genome walking has the potential to capture 6–7 kb of sequence in a single round. Ideal for identifying gene promoter regions where only the coding region. Genome walking also has significant utility for capturing homologous genes in new species when there are areas in the target gene with strong sequence conservation to the characterized species. The increasing use of next-generation sequencing technologies will see the principles of genome walking adapted to in silico methods. However, for smaller projects, PCR-based genome walking will remain an efficient method of characterizing unknown flanking sequence.