Title

A method for discovery of genome-wide SNP between any two genotypes from whole-genome re-sequencing data

Document Type

Book chapter

Publication details

Krishnan, SG, Waters, DLE & Henry, RJ 2014, 'A method for discovery of genome-wide SNP between any two genotypes from whole-genome re-sequencing data', Methods in Molecular Biology; vol. 1099, pp. 287-294.

Published version available from:

http://dx.doi.org/10.1007/978-1-62703-715-0_24

Abstract

Advances in sequencing technologies have aided the discovery of millions of genome-wide DNA polymorphisms such as single-nucleotide polymorphisms (SNPs) and insertion–deletions (InDels) which are an invaluable resource for marker-assisted breeding. Presently available bioinformatics tools assist the discovery of polymorphisms between target genotypes and the reference genome for a range of species. The discovery of polymorphisms between two genotypes within a breeding program is complicated by several factors such as bias in the number of reads from each genotype and residual heterozygosity within each genotype. In this chapter, we describe a novel approach where polymorphisms between a pair of genotypes are discovered from whole-genome re-sequencing data.