Title

The polymerase chain reaction (PCR): general methods

Document Type

Article

Publication details

Waters, DLE & Shapter, FM 2014, 'The polymerase chain reaction (PCR): general methods', Methods in Molecular Biology, vol. 1009, pp. 65-75.

Published version available from:

http://dx.doi.org/10.1007/978-1-62703-715-0_7

Abstract

The polymerase chain reaction (PCR) converts very low quantities of DNA into very high quantities and is the foundation of many specialized techniques of molecular biology. PCR utilizes components of the cellular machinery of mitotic cell division in vitro which respond predictably to user inputs. This chapter introduces the principles of PCR and discusses practical considerations from target sequence definition through to optimization and application.