Title

Plant defense response to fungal pathogens (activation of host-plasma membrane H+-ATPase by elicitor-induced enzyme dephosphorylation)

Document Type

Article

Publication details

Vera-Estrella, R, Barkla, BJ, Higgins, VJ & Blumwald, E 1994, 'Plant defense response to fungal pathogens (activation of host-plasma membrane H+-ATPase by elicitor-induced enzyme dephosphorylation)', Plant Physiology, vol. 104, no. 1, pp. 209-215.

Published version available from:

http://dx.doi.org/10.1104/pp.104.1.209

Peer Reviewed

Peer-Reviewed

Abstract

Elicitor preparations containing the avr5 gene products from race 4 of Cladosporium fulvum and tomato (Lycopersicon esculentum L.) cells near isogenic for the resistance gene Cf5 were used to investigate events following the treatment of host plasma membranes with elicitor. A 4-fold increase in H+-ATPase activity, coincident with the acidification of the extracellular medium, was detected immediately after elicitor treatment. The elicitor-induced stimulation of the plasma membrane H+-ATPase was inhibited by okadaic acid but not by staurosporine, suggesting that protein dephosphorylation was required for increased H+-ATPase activity. This observation was confirmed by [gamma]-32P labeling and immunodetection of the plasma membrane H+-ATPase. Effects of guanidine nucleotide analogs and mastoparan on the ATPase activity suggested the role of GTP-binding proteins in mediating the putative elicitor-receptor binding, resulting in activation of a phosphatase(s), which in turn stimulates the plasma membrane H+-ATPase by dephosphorylation.