Title

Bioactivity and chemical characterisation of Lophostemon suaveolens – an endemic Australian Aboriginal traditional medicinal plant

Document Type

Article

Publication details

Naz, T, Packer, J, Yin, P, Brophy, JJ, Wohlmuth, H, Renshaw, DE, Smith, J, Yaegl Community Elders, Vemulpad, SR & Jamie, JF 2016, 'Bioactivity and chemical characterisation of Lophostemon suaveolens – an endemic Australian Aboriginal traditional medicinal plant', Natural Product Research, vol. 30, no. 6, pp. 693-696.

Published version available from:

http://dx.doi.org/10.1080/14786419.2015.1038260

Peer Reviewed

Peer-Reviewed

Abstract

Lophostemon suaveolens is a relatively unexplored endemic medicinal plant of Australia. Extracts of fresh leaves of L. suaveolens obtained from sequential extraction with n-hexane and dichloromethane exhibited antibacterial activity in the disc diffusion and MTT microdilution assays against Streptococcus pyogenes and methicillin sensitive and resistant strains of Staphylococcus aureus (minimum bactericidal concentration < 63 μg/mL). The dichloromethane extract and chromatographic fractions therein inhibited nitric oxide in RAW264.7 murine macrophages (IC50 3.7–11.6 μg/mL) and also PGE2 in 3T3 murine fibroblasts (IC50 2.8–19.7 μg/mL). The crude n-hexane, dichloromethane and water extracts of the leaves and chromatographic fractions from the dichloromethane extract also showed modest antioxidant activity in the ORAC assay. GC–MS analysis of the n-hexane fraction showed the presence of the antibacterial compounds aromadendrene, spathulenol, β-caryophyllene, α-humulene and α-pinene and the anti-inflammatory compounds β-caryophyllene and spathulenol. Fractionation of the dichloromethane extract led to the isolation of eucalyptin and the known anti-inflammatory compound betulinic acid.