Identiﬁcation of major metabolites in Aloe littoralis by high-performance liquid chromatography–nuclear magnetic resonance spectroscopy
Karagianis, G, Viljoen, A & Waterman, PG 2003, 'Identiﬁcation of major metabolites in Aloe littoralis by high-performance liquid chromatography–nuclear magnetic resonance spectroscopy', Phytochemical Analysis, vol. 14, no. 5, pp. 275-280.
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Examination of the leaf exudate of the South African species Aloe littoralis by reversed-phase HPLC revealed the presence of two major metabolites. The identiﬁcation of the two compounds without isolation was attempted by HPLC-NMR based on separation using a C18 column eluting with a deuterium oxide:acetonitrile solvent gradient and an inverse HPLC-NMR probe. For each compound, one-dimensional proton spectra, and two-dimensional homonuclear COSY and TOCSY, and heteronuclear HSQC and HMBC, spectra were collected. On the basis of the data obtained, the metabolites were characterised as 10-hydroxyaloin B and deacetyllittoraloin.