An agar dilution method for the determination of the minimum inhibitory concentration of essential oils
Griffin, SG, Markham, JL & Leach, DN 2000, 'An agar dilution method for the determination of the minimum inhibitory concentration of essential oils', Journal of Essential Oil Research, vol. 12, no. 2, pp. 249-255.
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Abstract Methods for the measurement of the minimum inhibitory concentration (MIC) of antibacterial agents have been developed for water-soluble substances and require adaptation for use with water-insoluble essential oils. This paper reports a standardized agar dilution MIC method, using 0.5% v/v Tween 20 as a dispersant, which provides a reliable and reproducible technique. The method was tested using two Melaleuca alternifolia oil samples with two different levels of terpinen-4-ol (37% and 45% v/v). The MIC values of the tea tree oil samples against a wide selection of bacteria, moulds and yeast are reported.