Lymphocytes associated with adaptive immunity from the adenoids and peripheral blood of children from rural Australia and the correlation with chronic otitis media or adenoid hypertrophy
Browne, J, Matthews, E, Taylor-Robinson, A & Kyd, J 2015, 'Lymphocytes associated with adaptive immunity from the adenoids and peripheral blood of children from rural Australia and the correlation with chronic otitis media or adenoid hypertrophy', abstract presented to the 18th international symposium on recent advances in otitis media, Maryland, USA, 26 - 27 November.
Objective: Adenoidectomy is often used to manage chronic OM and how it contributes to improving OM is not well identified. This study aimed to identify factors that influence lymphocyte subsets from the blood and adenoids of children with and without chronic OM. It reports the association between adenoid and blood lymphocytes removed from children from an Australian rural community for either chronic OM or hypertrophy.
Method: 40 children aged 2 to7 years undergoing adenoidectomy in Rockhampton, Queensland were recruited. Background and clinical information was collected. B cell(CD19+), T cells(CD3+), T helper cells(CD3+CD4+), cytotoxic T cells(CD3+CD8+) and Treg cells(CD3+CD4+CD25hi+CD127lo+FoxP3+) were extracted from the blood and adenoids and analysed by multi-colour flow cytometry.
Results: Percentage of PBMC derived B cells cells (p<0.05) and Th cells (p<0.005) moderately, negatively correlated with age. Th cell percentages in children ≤3 years were higher than in children ≥4 years (p<0.005), and in children who did not attend daycare (p<0.005). PBMC derived Treg cells (p<0.05) had a moderate, positive correlation with daycare attendance. Age and daycare attendance did not influence lymphocyte percentages derived from the adenoid. Gender was significant for PBMC derived Tregs cells (p<0.05), but not in adenoid lymphocytes. Smoking exposure, birth order and number of children living in the household did not significantly influence lymphocyte subsets in the adenoid or blood.
Conclusion: In this population and within the two clinical groups, there were several moderate correlations associated with the child’s clinical condition. The lymphocyte populations in the adenoid were not deficient in the OM group.