Sesquiterpene lactone containing Mexican Indian medicinal plants and pure sesquiterpene lactones as potent inhibitors of transcription factor NF-ÎºB
Bork, PM, Schmitz, ML, Kuhnt, M, Escher, C & Heinrich, M 1997, 'Sesquiterpene lactone containing Mexican Indian medicinal plants and pure sesquiterpene lactones as potent inhibitors of transcription factor NF-ÎºB', FEBS Letters, vol. 402, no. 1, pp. 85-90.
The publisher's version of this article available at http://dx.doi.org/10.1016/S0014-5793(96)01502-5
The potential inhibitory effect of 54 Mexican Indian medicinal plants on the activation of transcription factor NF-ÎºB was studied. Band-shift experiments identified the ethanolic leaf extracts of Artemisia ludoviciana ssp. mexicana, Calea zacatechichi, and Polymnia maculata (all rich in sesquiterpene lactones) as inhibitors of NF-ÎºB down to a concentration of 25 Î¼g/ml. The sesquiterpene lactones isohelenin and parthenolide prevented NF-ÎºB activation completely as low as 5 Î¼M. Treatment of HeLa cells with leaf extract of A. ludoviciana ssp. mexicana, isohelenin and parthenolide prevented the induction of transcription on the IL-6 promoter. These experiments identify the eudesmanolide and germacranolide type of sesquiterpene lactones as potent non-antioxidant inhibitors of NF-ÎºB. All plants active in the NF-ÎºB assay also showed a delay in the onset of capillary reactions of the allantois membrane in a physiological model for anti-inflammatory activity - the HET-CAM assay.